• Product NameECI1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total ECI1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ECI1.
• Target NameECI1
• Alternative Names    ECI1; DCI; DodecenoylCoAisomerase; D3; D2enoylCoAisomerase
• Accession No. Swiss-Prot:P42126
  NCBI Gene ID:1632
• UniprotP42126
• Gene ID1632;
• SDS-PAGE MW 33KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described.

• Western blot analysis of extracts of various cell lines, using ECI1 at 1:1000 dilution.
• Immunofluorescence analysis of HeLa cells using ECI1 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting