• Product NameARHGAP25 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total ARHGAP25 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ARHGAP25.
• Target NameARHGAP25
• Alternative Names    ARHGAP25; KAIA0053;
• Accession No. Swiss-Prot:P42331
  NCBI Gene ID:9938
• UniprotP42331
• Gene ID9938;
• SDS-PAGE MW 73KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 ).

• Immunofluorescence analysis of Jurkat cells using ARHGAP25 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of L929 cells using ARHGAP25 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human kidney using ARHGAP25 at dilution of 1:200 (40x lens).
• Immunohistochemistry of paraffin-embedded Human gastric using ARHGAP25 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using ARHGAP25 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting