• Product NameATXN3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total ATXN3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ATXN3.
• Target NameATXN3
• Alternative Names    ATXN3; AT3; ATX3; JOS; MJD
• Accession No. Swiss-Prot:P54252
  NCBI Gene ID:4287
• UniprotP54252
• Gene ID4287;
• SDS-PAGE MW 43KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

• Confocal immunofluorescence analysis of U-2 OS cells using Ataxin 3 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using Ataxin 3 at 1:1000 dilution.
• Immunofluorescence analysis of HeLa cells using Ataxin 3 .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting