• Product Namep70 S6 Kinase(Ab-421) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total p70 S6 Kinase protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.419~423 (P-R-T-P-V) derived from Human p70 S6 Kinase.
• Target Namep70 S6 Kinase
• Alternative Names    KS6B1; P70-S6K; RPS6KB1; S6K;
• Accession No. Swiss-Prot: P23443
  NCBI Protein: NP_001258989.1
• UniprotP23443
• Gene ID6198;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 70 85 kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Xiao-Feng, et al. (2003) Le1 30 Volume 22: 484-497
An WL, et al. (2003) Am J Pathol. 163(2): 591-607.
Le XF, et al. (2003) Oncogene.22(4): 484-97

• Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-421) Antibody #21246(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Ab-421) Antibody #21246(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting