• Product NameSPP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total SPP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human Osteopontin (NP_001035147.1).
• Target NameSPP1
• Alternative Names    BNSP;BSPI;ETA-1;OPN;Osteopontin;SPP1
• Accession No. Uniprot:P10451
  GeneID:6696
• UniprotP10451
• Gene ID6696
• SDS-PAGE MW 65KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using Osteopontin antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting