• Product NamePRDX4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PRDX4 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PRDX4.
• Target NamePRDX4
• Alternative Names    AOE37-2; PRX-4;
• Accession No. Swiss-Prot:Q13162
  NCBI Gene ID:10549
• UniprotQ13162
• Gene ID10549;
• SDS-PAGE MW 31KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

PRDX4(Peroxiredoxin-4) is also named as AOE37-2, Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract, because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage(PMID:20864641). It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies(PMID:19208552). PRDX4 is a pentamer of dimers(PMID:23025503). This antibody is speific to PRDX4.

• Western blot analysis of extracts from normal (control) and PRDX4 knockout (KO) 293T cells, using PRDX4 at 1:1000 dilution.
• Western blot analysis of extracts of various cell lines, using PRDX4 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting