• Product NameEGFR(Ab-1110) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total EGFR protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 1108~1112 (P-V-Y-H-N) derived from Human EGFR.
• Target NameEGFR
• Alternative Names    Receptor tyrosine-protein kinase ErbB-1
• Accession No. Swiss-Prot: P00533
  NCBI Protein: NP_005219.2
• UniprotP00533
• Gene ID1956;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 175kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Kaisa Erjala, et al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111.
Judit Anido, et al. (2003) Clin. Cancer Res ; 9: 1274.
Julian Andreev, et al. (2001) J. Biol. Chem ; 276: 20130 - 20135.
Suil Kim, et al. (2002) Am J Physiol Lung Cell Mol Physiol; 283: 67.

• Western blot analysis of extracts from HUVEC and A431 cells using EGFR(Ab-1110) Antibody #21256.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-1110) Antibody #21256(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting