• Product NameSERPINF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total SERPINF1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human SERPINF1.
• Target NameSERPINF1
• Alternative Names    OI6; OI12; PEDF; EPC-1; PIG35
• Accession No. Swiss-Prot:P36955
  NCBI Gene ID:5176
• UniprotP36955
• Gene ID5176;
• SDS-PAGE MW 46KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

Pigment epithelium-derived growth factor (PEDF), also known as EPC-1 (early population doubling level cDNA-1), is a glycoprotein found naturally in the normal eye. PEDF has reported neuroprotective and differentiation properties and is secreted in abundance by retinal pigment epithelium cells. It belongs to the serine protease inhibitor (Serpin) superfamily and has been reported to inhibit angiogenesis and proliferation of several cell types. The ¡°pooling¡± of PEDF within the interphotoreceptor matrix places this molecule in a prime physical location to affect the underlying neural retina. Additionally, PEDF induces neuronal differentiation and promotes survival of neurons of the central nervous system from degeneration caused by serum withdrawal or glutamate cytotoxicity.

• Western blot analysis of extracts of various cell lines, using PEDF at 1:3000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting