• Product NamePOLB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total POLB protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human POLB.
• Target NamePOLB
• Alternative Names    MGC125976; POLB;
• Accession No. Swiss-Prot:P06746
  NCBI Gene ID:5423
• UniprotP06746
• Gene ID5423;
• SDS-PAGE MW 38KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:200 - 1:1000
  IHC 1:50 - 1:200
  IF 1:20 - 1:200

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.

• Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB at 1:1000 dilution.
• Western blot analysis of extracts of various cell lines, using POLB at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using POLB . Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded Human stomach using POLB at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded Mouse kidney using POLB at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting