• Product NameCYP11A1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total CYP11A1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human CYP11A1.
• Target NameCYP11A1
• Alternative Names    CYP11A; CYPXIA1; P450SCC;
• Accession No. Swiss-Prot:P05108
  NCBI Gene ID:1583
• UniprotP05108
• Gene ID1583;
• SDS-PAGE MW 60KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:20 - 1:50
  IF 1:20 - 1:50

The formation of biologically active steroid hormones is significantly influenced by the motrochondrial cytochrome P450 enzyme, chrolesterol side-chain cleavage. CYP11A1 functions in catalyzing the first step of steroid biosynthesis under modulation of cAMP.It ia also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al.

• Western blot analysis of extracts of various cell lines, using CYP11A1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human placenta using CYP11A1 at dilution of 1:150 (40x lens).
• Immunofluorescence analysis of NIH/3T3 cells using CYP11A1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting