• Product NameNR0B1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total NR0B1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human NR0B1.
• Target NameNR0B1
• Alternative Names    AHC; AHX; DSS; GTD; HHG
• Accession No. Swiss-Prot:P51843
  NCBI Gene ID:190
• UniprotP51843
• Gene ID190;
• SDS-PAGE MW 52KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:10 - 1:100

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

• Western blot analysis of extracts of various cell lines, using NR0B1 at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using NR0B1 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting