• Product Namep70 S6 Kinase(Ab-411) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total p70 S6 Kinase protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 409~413 (I-R-S-P-R) derived from Human p70 S6 Kinase.
• Target Namep70 S6 Kinase
• Alternative Names    KS6B1; P70-S6K; RPS6KB1; S6K;
• Accession No. Swiss-Prot: P23443
  NCBI Protein: NP_003152.1
• UniprotP23443
• Gene ID6198;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 70 85 kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484

• Western blot analysis of extracts from 293 cells using p70 S6 Kinase(Ab-411) Antibody #21261.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase(Ab-411) Antibody #21261(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase(Ab-411) Antibody #21261.
• Western blotting analysis using p70 S6 Kinase(Ab-411) Antibody #21261.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting