• Product NameICOS Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total ICOS protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ICOS.
• Target NameICOS
• Alternative Names    AILIM; CD278; CVID1; MGC39850;
• Accession No. Swiss-Prot:Q9Y6W8
  NCBI Gene ID:29851
• UniprotQ9Y6W8
• Gene ID29851;
• SDS-PAGE MW 23KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

• Immunofluorescence analysis of rat thymus using ICOS at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of mouse thymus using ICOS at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using ICOS at 1:1000 dilution.
• Immunofluorescence analysis of human spleen using ICOS at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting