• Product NameEIF6 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total EIF6 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human EIF6.
• Target NameEIF6
• Alternative Names    CAB; EIF3A; eIF-6; p27BBP; ITGB4BP
• Accession No. Swiss-Prot:P56537
  NCBI Gene ID:3692
• UniprotP56537
• Gene ID3692;
• SDS-PAGE MW 27KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3).

• Western blot analysis of extracts of various cell lines, using EIF6 at 1:1000 dilution.
• Confocal immunofluorescence analysis of Hela cells using EIF6 at dilution of 1:50. Blue: DAPI for nuclear staining.
• Confocal immunofluorescence analysis of U-2OS cells using EIF6 at dilution of 1:50. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of MCF-7 cells using EIF6 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting