• Product NameeIF2a(Ab-51) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total eIF2a protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2a.
• Target NameeIF2a
• Alternative Names    Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A;
• Accession No. Swiss-Prot: P05198
  NCBI Protein: NP_004085.1
• UniprotP05198
• Gene ID1965;
• Concentration 0.6mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 38kd
  Western blotting: 1:200~1:1000
  Immunohistochemistry: 1:30~1:150
   

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hong-Li Wu, Yu-Hua Li, Yan-Hua Lin, et al. (2008) Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction Cardiovasc Res doi:10.1093/cvr/cvn262
This article references the use of the #21271.

• Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-6: A549 cell,Human placenta tissue,A172 cell,NIH/3T3 cell,Jurkat cell,Hela cell lysates Primary antibody: 21271(eIF2a Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes
• The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 21271(eIF2a(Ab-51) Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
• The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 21271(EIF2S1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting