• Product NameIntegrin b3(Ab-785) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Integrin b3 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.783~787 (I-T-Y-R-G) derived from Human Integrin B3/ITGB3.
• Target NameIntegrin b3
• Alternative Names    CD61 antigen; GP3A; GPIIIa; ITB3; Platelet membrane glycoprotein IIIa
• Accession No. Swiss-Prot: P05106
  NCBI Protein: NP_000203.2
• UniprotP05106
• Gene ID3690;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 110kd
  Western blotting: 1:500~1:1000

The Itg-Beta3 gene encodes the beta subunit of the platelet membrane adhesive protein receptor complex Itg-Beta3 protein, which belongs to the Integrin class of cell adhesion molecule receptors that share a common heterotrimeric structure with a and Beta subunits. It is a common Beta subunit of the platelet complex and of two other Integrins (Fibronectin and Vitronectin Receptors), which have distinct a subunits. Itg-Beta3 has been implicated in a wide variety of functions, including platelet aggregation and thrombosis and implantation, placentation, angiogenesis, bone remodeling, and tumor progression. Glanzmann Thrombasthenia can result from defects in the genes for either the Itg-a2B or the Itg-Beta3 subunit

Sujoy Bhattacharya, et al. (2006) Biochem J. August 1; 397(Pt 3): 437

• Western blot analysis of extracts from HL60 and HT29 cells using Integrin b3(Ab-785) Antibody #21274.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting