• Product NameHSD17B2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total HSD17B2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human HSD17B2.
• Target NameHSD17B2
• Alternative Names    HSD17; SDR9C2; EDH17B2;
• Accession No. Swiss-Prot:P37059
  NCBI Gene ID:3294
• UniprotP37059
• Gene ID3294;
• SDS-PAGE MW 42KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  Immunohistochemistry: 1:50 - 1:200
  Immunofluorescence: 1:50 - 1:200

Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.

• Western blot analysis of extracts of various cell lines, using HSD17B2 antibody.
• Immunofluorescence analysis of U2OS cell using HSD17B2 antibody. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat brain using HSD17B2 antibody at dilution of 1:200 (x400 lens).
• Immunohistochemistry of paraffin-embedded Human gastric using HSD17B2 antibody at dilution of 1:100 (x400 lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting