• Product NameEIF5A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total EIF5A protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human EIF5A.
• Target NameEIF5A
• Alternative Names    EIF-5A; EIF5A1; eIF5AI;
• Accession No. Swiss-Prot:P63241
  NCBI Gene ID:1984
• UniprotP63241
• Gene ID1984;
• SDS-PAGE MW 16KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

mRNA-binding protein involved in translation elongation. Has an important function at the level of Mrna turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.

• Western blot analysis of extracts of various cell lines, using EIF5A at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded human kidney using EIF5A at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of U2OS cells using EIF5A . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting