• Product NameBIM(Ab-69) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total BIM protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.67~71 (P-A-S-P-G) derived from Human BIM.
• Target NameBIM
• Alternative Names    BCL2-like protein 11; BCL2L11; BOD; Bcl-2 related ovarian death protein;
• Accession No. Swiss-Prot: O43521
  NCBI Protein: NP_006529.1
• UniprotO43521
• Gene ID10018;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 23kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-a1, isoform Bim-a2 and isoform Bim-a3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.

Fukazawa H, et al. (2004) Mol Cancer Ther ; 3(10): 1281-1288
Luciano F, et al. (2003) Oncogene ; 22(43): 6785-6793

• Western blot analysis of extracts from Hela cells using BIM(Ab-69) Antibody #21280.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM(Ab-69) Antibody #21280(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting