NFkB-p65(Phospho-Ser536) Antibody - SAB

Catalog No.	Package Size	Price	Quantity
11014-1	50ul	$219.00
11014-2	100ul	$319.00

Catalog No.

Package Size

Price

Quantity

11014-1

50ul

$219.00

11014-2

100ul

$319.00

Product NameNFkB-p65(Phospho-Ser536) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsIF;WB;IHC;IP
Species ReactivityHuman;Mouse;Rat;Monkey
SpecificityThe antibody detects endogenous level of NF-kB p65 only when phosphorylated at serine 536.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of serine 536 (F-S-S(p)-I-A) derived from Human NFkB-p65.
Target NameNFkB-p65
ModificationPhospho
Alternative Names NFKB3; RELA; TF65; Transcription factor p65; p65

Accession No. Swiss-Prot: Q04206
NCBI Protein: NP_001138610.1
UniprotQ04206
Gene ID5970;
Concentration 1.0mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate.

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

Doyle S L, et al. (2005) J Biol Chem. 280(25): 23496-23501.
Anwar K N, et al. (2004) J Immunol. 173(11): 6965-6972.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.

NFkB-p65(Phospho-Ser536) Antibody #11014

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