• Product NameSynaptotagmin 1/2 (Ab-202/199) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total Synaptotagmin 1/2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.200~204/197~201 (R-K-T-L-N) derived from Human Synaptotagmin 1/2.
• Target NameSynaptotagmin 1/2
• Alternative Names    SYT1/2; SytI/II; Synaptotagmin I/II; P65; SYT
• Accession No. Swiss-Prot:P21579
  Gene ID:6857
• UniprotP21579
• Gene ID6857;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 46kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse

Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7.
Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31.
Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.

• Western blot analysis of extracts from Hela cells using Synaptotagmin 1/2(Ab-202/199) Antibody #21293 and the same antibody preincubated with blocking peptide.
• Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1/2(Ab-202/199) Antibody #21293.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting