• Product NameIkB-e(Ab-22) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total IkB-e protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 20~24 (I-E-S-L-R) derived from Human IkB-e.
• Target NameIkB-e
• Alternative Names    I-kappa-B-epsilon
• Accession No. Swiss-Prot: O00221
  NCBI Protein: NP_004547.2
• UniprotO00221
• Gene ID4794;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 40kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.

• Western blot analysis of extracts from Hela, 293 and HepG2 cells using IkB-e(Ab-22) Antibody #21296.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Ab-22) Antibody #21296(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting