• Product NameNFkB-p100(Ab-872) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total NFkB-p100 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.870~874 (S-Q-S-V-E) derived from Human NFkB-p100.
• Target NameNFkB-p100
• Alternative Names    DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; NFKB2
• Accession No. Swiss-Prot: Q00653
  NCBI Protein: NP_001070961.1
• UniprotQ00653
• Gene ID4791;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 120 kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Shin HM, et al. (2006) EMBO J; 25(1): 129-138.
Li Q, et al. (2005) Proc Natl Acad Sci USA; 102(35): 12425-12430.
Chen C, et al. (2000) Mol Cell Biol; 20(8): 2687-2695.
 

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFkB-p100/p52(Ab-872) Antibody #21297(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p100(Ab-872) Antibody #21297.
• Western blot analysis of extracts from HT29 cells using NFkB-p100(Ab-872) Antibody #21297 and the same antibody preincubated with blocking peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting