• Product NameTBK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IHC
• Species ReactivityHuman,Rat,Mouse
• SpecificityThe antibody detects endogenous level of total TBK1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human TBK1.
• Target NameTBK1
• Alternative Names    NAK; T2K;
• Accession No. Swiss-Prot:Q9UHD2
  NCBI Gene ID:29110
• UniprotQ9UHD2
• Gene ID29110;
• SDS-PAGE MW 84KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

  IHC 1:50 - 1:200

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors.

• Western blot analysis of extracts of Mouse brain, using TBK1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat testis using TBK1/NAK Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting