• Product NameRel(Phospho-Ser503) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Rel only when phosphorylated at serine 503.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 503 (T-S-S(p)-D-S) derived from Human Rel.
• Target NameRel
• ModificationPhospho
• Alternative Names    C-Rel
• Accession No. Swiss-Prot: Q04864
  NCBI Protein: NP_002899.1
• UniprotQ04864
• Gene ID5966;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 78kd
  Immunofluorescence: 1:100~1:200

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 (PubMed 18037997)).

Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
Haskill, S. et al. (1991) Cell 65, 1281-9.
Thompson, J.E. et al. (1995) Cell 80, 573-82.

• Immunofluorescence staining of methanol-fixed Hela cells using Rel(Phospho-Ser503) Antibody #11020.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting