• Product NameGSK3α/β(Ab-279/216) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total GSK3α/β protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.277~281/214~218 (V-S-Y-I-C) derived from Human GSK3α/β.
• Target NameGSK3α/β
• Alternative Names    Factor A; GSK-3 alpha/beta; kinase GSK3-alpha/beta
• Accession No. Swiss-Prot:P49840
  Gene ID:2931
• UniprotP49840
• Gene ID2931;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 46, 51kd
  Western blotting: 1:500~1:1000

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137(1-2): 193-201
Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316(2): 411-415
Fan G, et al. J Biol Chem 2003 Dec 26; 278(52): 52432-52436
Liao X, et al. Mol Cancer Ther 2003 Nov; 2(11): 1215-1222

• Western blot analysis of extracts from HL60, HT29 and 293 cells using GSK3α/β(Ab-279/216) Antibody #21301.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting