• Product NameCYB5A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total CYB5A protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human CYB5A.
• Target NameCYB5A
• Alternative Names    CYB5; MCB5;
• Accession No. Swiss-Prot:P00167
  NCBI Gene ID:1528
• UniprotP00167
• Gene ID1528;
• SDS-PAGE MW 15KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using CYB5A at 1:1000 dilution.
• Immunofluorescence analysis of C6 cells using CYB5A Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat kidney using CYB5A at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse heart using CYB5A at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting