• Product NameNDC80 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total NDC80 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human NDC80.
• Target NameNDC80
• Alternative Names    HEC; HEC1; TID3; KNTC2; HsHec1
• Accession No. Swiss-Prot:O14777
  NCBI Gene ID:10403
• UniprotO14777
• Gene ID10403;
• SDS-PAGE MW 74KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

• Western blot analysis of extracts of various cell lines, using NDC80 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting