• Product NameSHP-2(Ab-542) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was purified by immunogen affinity chromatography.
• ApplicationsWB, IHC, IF/ICC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of SHP-2 protein.
• Immunogen TypePeptide-KLH
• Immunogen Description KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SHPTP2. The exact sequence is proprietary.
• Target NameSHP-2
• Alternative Names    Protein-tyrosine phosphatase 2C
• Accession No. Swiss-Prot: Q06124
  NCBI Protein: NP_002825.3
• UniprotQ06124
• Gene ID5781;
• Concentration 1mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• WB 1/500 - 1/1000, IHC 1/50 - 1/100, IF/ICC 1/50 - 1/200

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14(9): 3911-3928.
Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536.
Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682
Manes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135.
Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.
 

• Western blot analysis of SHPTP2 expression in C6 (A), SGC7901(B), H1792 (C), SKOVCAR3 (D), A2780 (E), Hela (F) whole celllysates.
• Immunohistochemical analysis of SHPTP2 staining in humanbreast cancer formalin fixed paraffin embedded tissue section.The section was pre-treated using heat mediated antigenretrieval with sodium citrate buffer (pH 6.0). The section wasthen incubated with the antibody at room temperature anddetected using an HRP conjugated compact polymer system.DAB was used as the chromogen. The section was thencounterstained with haematoxylin and mounted with DPX.
• Immunofluorescent analysis of SHPTP2 staining in SGC7901cells. Formalin-fixed cells were permeabilized with 0.1% TritonX-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBSfor 30 minutes at room temperature. Cells were probed withthe primary antibody in 3% BSA-PBS and incubated overnightat 4 °C in a hidified chamber. Cells were washed with PBST andincubated with a AF488-conjugated secondary antibody(green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was usedto stain the cell nuclei (blue).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting