• Product NameSHP-2(Ab-580) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total SHP-2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.578~582 (R-V-Y-E-N) derived from Human SHP-2.
• Target NameSHP-2
• Alternative Names    Protein-tyrosine phosphatase 2C
• Accession No. Swiss-Prot: Q06124
  NCBI Protein: NP_002825.3
• UniprotQ06124
• Gene ID5781;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 72kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14(9): 3911-3928.
Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536.

• Western blot analysis of extracts from 293 cells using SHP-2(Ab-580) Antibody #21320.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2(Ab-580) Antibody #21320(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using SHP-2(Ab-580) Antibody #21320.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting