• Product NamePRPF3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PRPF3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PRPF3.
• Target NamePRPF3
• Alternative Names    PRP3; RP18; HPRP3; Prp3p; HPRP3P
• Accession No. Swiss-Prot:O43395
  NCBI Gene ID:9129
• UniprotO43395
• Gene ID9129;
• SDS-PAGE MW 78KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200
  IP 1:50 - 1:100

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.

• Western blot analysis of extracts of various cell lines, using PRPF3 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat brain using PRPF3 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using PRPF3 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using PRPF3 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of U2OS cells using PRPF3 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting