• Product NameDNAJB1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total DNAJB1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human DNAJB1.
• Target NameDNAJB1
• Alternative Names    Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Accession No. Swiss-Prot:P25685
  NCBI Gene ID:3337
• UniprotP25685
• Gene ID3337;
• SDS-PAGE MW 38KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

• Immunofluorescence analysis of U2OS cells using DNAJB1 . Blue: DAPI for nuclear staining.
• Western blot analysis of extracts from normal (control) and DNAJB1 knockout (KO) 293T cells, using DNAJB1 at 1:1000 dilution.
• Immunofluorescence analysis of C6 cells using DNAJB1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of HeLa cells using DNAJB1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using DNAJB1 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting