• Product NamePGRMC1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PGRMC1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PGRMC1.
• Target NamePGRMC1
• Alternative Names    MPR; HPR6.6;
• Accession No. Swiss-Prot:O00264
  NCBI Gene ID:10857
• UniprotO00264
• Gene ID10857;
• SDS-PAGE MW 21KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene encodes a putative membrane-associated progesterone steroid receptor. The protein is expressed predominantly in the liver and kidney.

• Immunofluorescence analysis of C6 cells using PGRMC1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human tonsil using PGRMC1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human vermiform appendix using PGRMC1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using PGRMC1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of L929 cells using PGRMC1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using PGRMC1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using PGRMC1 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded rat kidney using PGRMC1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting