• Product NameROCK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total ROCK2 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human ROCK2 (NP_004841.2).
• Target NameROCK2
• Alternative Names    ROCK2;ROCK-II
• Accession No. Uniprot:O75116
  GeneID:9475
• UniprotO75116
• Gene ID9475
• SDS-PAGE MW 161KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

• Western blot analysis of extracts of various cell lines, using ROCK2 antibody.
• Western blot analysis of extracts of various cell lines, using ROCK2 antibody.
• Immunohistochemistry of paraffin-embedded rat brain using ROCK2 Rabbit pAb.
• Immunohistochemistry of paraffin-embedded human colon carcinoma using ROCK2 Rabbit pAb.
• Immunofluorescence analysis of HeLa cells using ROCK2 Rabbit pAb.
• Immunofluorescence analysis of HepG2 cells using ROCK2 Rabbit pAb.
• Immunofluorescence analysis of PC-12 cells using ROCK2 Rabbit pAb.
• Immunohistochemistry of paraffin-embedded mouse pancreas using ROCK2 Rabbit pAb.
• Immunofluorescence analysis of NIH/3T3 cells using ROCK2 Rabbit pAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting