• Product NameETV4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total ETV4 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ETV4.
• Target NameETV4
• Alternative Names    E1AF; PEA3; E1A-F; PEAS3;
• Accession No. Swiss-Prot:P43268
  NCBI Gene ID:2118
• UniprotP43268
• Gene ID2118;
• SDS-PAGE MW 53KD
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  Immunohistochemistry: 1:50 - 1:200
  Immunofluorescence: 1:50 - 1:200

Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.

• Western blot analysis of extracts of various cell lines, using Pea3 / ETV4 antibody at 1:1000 dilution.
• Immunofluorescence analysis of U2OS cells using Pea3 / ETV4 antibody. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting