• Product Namecofilin1/cofilin2(Ab-88) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total cofilin1/cofilin2 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human cofilin1/cofilin2.
• Target Namecofilin1/cofilin2
• Alternative Names    Cofilin; non-muscle isoform 18 kDa phosphoprotein;
• Accession No. Swiss-Prot:P23528
  Gene ID:1072
• UniprotP23528
• Gene ID1072;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 19kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
Arber, S. et al. (1998) Nature 393, 805-809.
Yang, N. et al. (1998) Nature 393, 809-812.

• Western blot analysis of extracts from MDA cells using cofilin1/cofilin2(Ab-88) Antibody #21507.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin1/cofilin2(Ab-88) Antibody #21507(left) or the same antibody preincubated with blocking peptide(right).
• Immunofluorescence staining of methanol-fixed Hela cells using cofilin1/cofilin2(Ab-88) Antibody #21507.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting