• Product NameNuclear Receptor NR4A1 (Ab-351) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Nuclear Receptor NR4A1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of serine 351 (L-P-S(p)-K-P).
• Target NameNuclear Receptor NR4A1
• Alternative Names    Early response protein NAK1; GFRP; GFRP1; HMR; N10
• Accession No. Swiss-Prot: P22736
  NCBI Gene ID: 3164
• UniprotP22736
• Gene ID3164;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' By similarity. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.

Nakai A., Mol. Endocrinol. 4:1438-1443(1990).
Chang C., J. Steroid Biochem. 34:391-395(1989).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Ab-351) antibody #33181.
• Western blot analysis of extracts from COS-7 cells, using Nuclear Receptor NR4A1 (Ab-351) antibody #33181.
• Western blot analysis of extracts from HepG2 cells (Lane 2), using Nuclear Receptor NR4A1 (Ab-351) antiobdy #33181. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting