• Product NameANXA1 (Ab-21) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ANXA1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized non-phosphopeptide derived from human ANXA1 around the phosphorylation site of tyrosine 21 (Q-E-Y(p)-V-Q).
• Target NameANXA1
• Alternative Names    annexin A1; Annexin I; ANX1; Calpactin II; Chromobindin 9
• Accession No. Swiss-Prot: P04083
  NCBI Gene ID: 301
• UniprotP04083
• Gene ID301;
• SDS-PAGE MW 39kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Wallner B.P., Nature 320:77-81(1986).
Kovacic R.T., Biochemistry 30:9015-9021(1991).
Arcone R., Eur. J. Biochem. 211:347-355(1993)

• Western blot analysis of extracts from 293 cells, using ANXA1 (Ab-21) antibody #33272.
• Western blot analysis of extracts from JK cells (Lane 2), K562 cells (Lane 3) and 293 cells (Lane 4), using ANXA1 (Ab-21) antiobdy #33272. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting