• Product Name53BP1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total 53BP1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human 53BP1.
• Target Name53BP1
• Alternative Names    p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1
• Accession No. Swiss-Prot: Q12888
  NCBI Gene ID: 7158
• UniprotQ12888
• Gene ID7158;
• SDS-PAGE MW 213kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  

Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation.

Iwabuchi K., J. Biol. Chem. 273:26061-26068(1998).
The German cDNA consortium; Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
Nakajima D., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

• Immunohistochemistry analysis of paraffin-embedded human brain tissue using 53BP1 antibody #33297.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting