• Product NamePaxillin(Ab-88) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Paxillin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.86~90 (P-V-Y-G-S) derived from Human Paxillin.
• Target NamePaxillin
• Alternative Names    PAXI; PXN;
• Accession No. Swiss-Prot: P49023
  NCBI Protein: NP_001074324.1
• UniprotP49023
• Gene ID5829;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 68kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:50~1:100

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441
Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.

• Western blot analysis of extracts from HUVEC cells using Paxillin(Ab-88) Antibody #21538 and the same antibody preincubated with blocking peptide.
• Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(Ab-88) Antibody #21538.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting