• Product NameBax Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Rt
• SpecificityThe antibody detects endogenous levels of total Bax protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Bax.
• Target NameBax
• Alternative Names    Apoptosis regulator BAX; membrane isoform alpha; BAXA; Bcl2-associated X protein;
• Accession No. Swiss-Prot: Q07812
  NCBI Gene ID: 581/581
• UniprotQ07812
• Gene ID581;
• SDS-PAGE MW 21kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Francesca Andriani, J Natl Cancer Inst, Sep 2001; 93: 1314 - 1324.
H Martinez-Valdez, J. Exp. Med., Mar 1996; 183: 971.
Joslyn K. Brunelle, J. Biol. Chem., Feb 2004; 279: 4305 - 4312.
Renren Wen, Mol. Cell. Biol., Jan 2001; 21: 678 - 689.

• Western blot analysis of extracts from 293 cells, using Bax antibody #33327.
• Immunohistochemical analysis of paraffin-embedded human brain tissue using Bax antibody #33327.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting