• Product NameCollagen III Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total collagen III protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human collagen III.
• Target NameCollagen III
• Alternative Names    Collagen alpha 1; Collagen alpha 1(III) chain [Precursor];
• Accession No. Swiss-Prot: P02461
  NCBI Gene ID: 1281
• UniprotP02461
• Gene ID1281;
• SDS-PAGE MW 138kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Arja Lamberg, J. Biol. Chem., May 1996; 271: 11988.
GW Conrad, J. Cell Biol., Mar 1980; 84: 501.
DR Keene, J. Cell Biol., Nov 1987; 105: 2393.
HM Wang, J. Histochem. Cytochem., Nov 1980; 28: 1215.

• Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using Collagen III antibody #33341.
• Immunofluorescence analysis of HeLa cells, using Collagen III antibody #33341.
• Western blot analysis of extracts from Hela, using Collagen III Antibody.,The lane on the left was treated with the antigen-specific peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting