• Product NameConnexin 43 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Connexin 43 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human Connexin 43.
• Target NameConnexin 43
• Alternative Names    CX43; CXA1; CXN-43; GJA1; Gap junction 43 kDa heart protein
• Accession No. Swiss-Prot: P17302
  NCBI Gene ID: 2697
• UniprotP17302
• Gene ID2697;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract By similarity.

Toshihiko Toyofuku, J. Biol. Chem., May 1998; 273: 12725.
Toshihiko Toyofuku, J. Biol. Chem., Jan 1998; 273: 1519.
Masayuki Shimada, Biol Reprod, Apr 2001; 64: 1255.
Merry L. Lindsey, Circulation, Jun 2006; 113: 2919 - 2928.

• Immunohistochemical analysis of paraffin-embedded human brain tissue using Connexin 43 antibody #33343.
• Immunofluorescence analysis of A549 cells, Connexin 43 antibody #33343.
• Western blot analysis of extracts from A549 cells, using Connexin 43 antibody #33343.
• Western blot analysis of extracts from 3T3 cells (Lane 2), using Connexin 43 antiobdy #33343. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting