• Product NameFAK(Ab-576/577) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total FAK protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.574~578/575~579 (T-Y-Y-K-A) derived from Human FAK.
• Target NameFAK
• Alternative Names    FADK 1; FAK1; PTK2
• Accession No. Swiss-Prot: Q05397
  NCBI Protein: NP _005598.3
• UniprotQ05397
• Gene ID5747;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 125kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.

• Western blot analysis of extracts from HT29 and Hela cells using FAK(Ab-576/577) Antibody #21545.
• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK(Ab-576/577) Antibody #21545(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting