• Product NameGRP78 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total GRP78 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human GRP78.
• Target NameGRP78
• Alternative Names    BIP; GLUCOSE-REGULATED PROTEIN 78-KD; HEAT-SHOCK 70-KD PROTEIN 5; IMMUNOGLOBULIN HEAVY CHAIN-BINDING PROTEIN; heat shock 70kDa protein 5 (glucose-regulated protein
• Accession No. Swiss-Prot: P11021
  NCBI Gene ID: 3309
• UniprotP11021
• Gene ID3309;
• SDS-PAGE MW 75kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Uma K. Misra, J. Biol. Chem., Oct 2002; 277: 42082 - 42087.
CA Clairmont, J. Biol. Chem., Feb 1992; 267: 3983 - 3990.
J Tooze, J. Cell Biol., Jul 1989; 109: 35.
Jong K. Yun, PNAS, Dec 1997; 94: 13903.

• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GRP78 antibody #33395.
• Immunofluorescence analysis of COS7 cells, using GRP78 antibody #33395.
• Western blot analysis of extracts from HUVEC, HepG2 and Hela cells, using GRP78 antibody #33395.
• Western blot analysis of extracts from HuvEc cells (Lane 2), COS7 cells (Lane 3) and JK cells (Lane 4), using GRP78 antiobdy #33395. The lane on the left is treated with systhesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting