• Product Namec-Cbl(Ab-700) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total c-Cbl protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 698~702 (T-E-Y-M-T) derived from Human c-Cbl.
• Target Namec-Cbl
• Alternative Names    Signal transduction protein CBL; Proto-oncogene c-CBL; Casitas B-lineage lymphoma proto-oncogene; RING finger protein 55;
• Accession No. Swiss-Prot: P22681
  NCBI Protein: NP _005179.2
• UniprotP22681
• Gene ID867;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 120kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

Blake, T.J. et al. (1991) Oncogene 6, 653-657.
Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.

• Western blot analysis of extracts from Hela and HT29 cells using c-Cbl(Ab-700) Antibody #21549.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl(Ab-700) Antibody #21549(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting