• Product NameJunB(Phospho-Ser259) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of JunB only when phosphorylated at serine 259.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB.
• Target NameJunB
• ModificationPhospho
• Alternative Names    AP-1
• Accession No. Swiss-Prot: P17275
  NCBI Protein: NP_002220.1
• UniprotP17275
• Gene ID3726;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 43kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.

• Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB(Phospho-Ser259) Antibody #11027.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Phospho-Ser259) Antibody #11027(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting