• Product NameMyc-Tag Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.9E10
• IsotypeIgG1
• PurificationAntibodies were produced from mice ascites by injecting mice with a monoclonal cell line which was fusioned by mouse spleen and SP2/0 myeloma cell. Spleen cells were isolated from mice by immunizing with synthetic peptide and KLH conjugates.
• ApplicationsWB
• SpecificityThe mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected cells.
• Immunogen TypePeptide
• Immunogen DescriptionPeptide sequence around aa. 410-419 (EQKLISEEDL) derived from human c-Myc.
• Target NameMyc-Tag
• Accession No. NCBI Protein: NP_002458.2
• Concentration 1.0mg/ml
• Formulation Supplied in mice ascites
• Storage Store at 4°C for short term use. Store at -20°C for long term preservation. Avoid freezing and thawing repeatly.

• Western blotting: 1:5000~1:10000
   

Epitope tags are artificial epitopes useful for the labeling and detection of proteins. Epitope tags - short amino acid sequences are 'fused' to the N- or C-terminus of the protein.

Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

• Western blot analysis of extracts from 293 cells transfected with Myc-CDK2 using Myc-Tag mouse mAb #T504.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting