• Product NameNRF2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total NRF2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from human NRF2.
• Target NameNRF2
• Alternative Names    HEBP1; NF-E2 related factor 2; NF2L2; NFE2-related factor 2; NFE2L2
• Accession No. Swiss-Prot: Q16236
  NCBI Gene ID: 4780
• UniprotQ16236
• Gene ID4780;
• SDS-PAGE MW 68kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Rajesh K. Thimmulappa, J. Clin. Invest., Apr 2006; 116: 984 - 995.
Sara B. Cullinan, Mol. Cell. Biol., Oct 2003; 23: 7198 - 7209.
Tirumalai Rangasamy, J. Exp. Med., Jul 2005; 202: 47 - 59.
Wenge Li, J. Biol. Chem., Aug 2005; 280: 28430 - 28438.

• Western blot analysis of extracts from HuvEc cells, using NRF2 antibody #33450.
• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NRF2 antibody #33450.
• Immunofluorescence analysis of HuvEc cells, using NRF2 antibody #33450.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting