• Product NameCaspase 9 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Caspase 9 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9
• Target NameCaspase 9
• Alternative Names    ICE-like apoptotic protease 6
• Accession No. Swiss-Prot: P55211
  NCBI Protein: NP_001220.2
• UniprotP55211
• Gene ID842;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 47 37 35kd
  Western blotting: 1:1000
  

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
Li, P. et al. (1997) Cell 91, 479-489.

• Western blot analysis of extracts from 293 and Hela cells using Caspase 9 Antibody #21422.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting